Description
Key Components:
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Vitamin D₂ (Ergocalciferol): Obtained from plant-based foods and fortified products.
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Vitamin D₃ (Cholecalciferol): Produced naturally in the skin when exposed to sunlight and found in animal-based foods.
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The test measures 25-hydroxyvitamin D [25(OH)D], which is the main form circulating in the blood and the most accurate indicator of Vitamin D status.
Uses:
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To detect Vitamin D deficiency or insufficiency.
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To monitor Vitamin D supplementation therapy.
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To evaluate bone health and detect conditions like osteoporosis, osteomalacia, or rickets.
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To support diagnosis of calcium imbalance or parathyroid gland disorders.
Benefits:
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Helps identify deficiency early to prevent bone weakness, fatigue, and immune issues.
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Supports the management of chronic conditions related to bone and muscle health.
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Provides insights into overall metabolic and hormonal balance.
How It Works:
A blood sample is drawn from a vein, and the 25-hydroxy Vitamin D level is analyzed using advanced immunoassay or chromatography methods. The result reflects your total Vitamin D status from both diet and sunlight exposure.
Normal Range (May Vary by Lab):
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Deficient: < 20 ng/mL
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Insufficient: 20–29 ng/mL
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Sufficient: 30–100 ng/mL
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Possible Toxicity: > 100 ng/mL
Preparation Before Test:
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Fasting: Not required.
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Medication: Inform your doctor about Vitamin D or calcium supplements, as they can affect results.
Warnings & Precautions:
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Excessive Vitamin D supplementation can lead to toxicity, causing high calcium levels (hypercalcemia).
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Always consult your doctor before starting or changing Vitamin D dosage.
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Levels can vary seasonally depending on sunlight exposure.
Synopsis:
The Vitamin D, Total test is a vital health assessment tool that provides an accurate measure of your body’s Vitamin D levels. It helps maintain strong bones, boosts immunity, and supports overall wellness through early detection of deficiencies or excesses.
FAQs About Vitamin D, Total
Q1. Why is this test important?
Because Vitamin D supports calcium absorption, bone strength, and immune defense.
Q2. Do I need to fast before this test?
No fasting is needed.
Q3. What causes Vitamin D deficiency?
Lack of sunlight, poor diet, limited outdoor activity, or certain medical conditions.
Q4. How often should I get tested?
As advised by your doctor, usually once or twice a year, especially if you’re at risk of deficiency or on supplements.
Test requirements
- Fasting Not Required
- Blood Sample

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